SEQUENCING
The Hugh Green Technology Centre utilises the MinION and PromethION P2 Solo from Oxford Nanopore Technologies for long-read sequencing.
OXFORD NANOPORE LONG-READ SEQUENCING
We use the MinION and PromethION P2 Solo from Oxford Nanopore Technologies for long-read sequencing.
Nanopore sequencing involves passing a long polymer through a protein pore using an electric current to detect changes in translocation speed, which vary based on the DNA base. This rapid, continuous method allows for efficient DNA sequencing.
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The MinION and PromethION P2 Solo sequencers use consumable flow cells with 2,048 sequencing wells (MinION). Each well has a polymer membrane with embedded protein pores. An electrical potential across the membrane drives ion flow through the nanopore, sequencing DNA.​
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​At any time, 512 wells (MinION) are connected to sensing circuits, capturing 4,000 snapshots per second (4 kHz) and measuring up to 450 DNA bases per second, yielding up to 40 gigabases from a two-day run.
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MinION Flow Cell: up to 50 million Gb of data
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PromethION Flow Cell: up to 290 million Gb of dat
A symbolic representation of a chimeric read captured by an ONT MinION DNA sequencer (A), together with the annotated raw signal (B), containing three separate hairpin events within the same base-called sequence. Hairpin features can be seen by eye within the signal; attached barcodes demonstrate that this sequence was formed from an overnight ligation reaction. White R., et al. F1000 Research 2017.